| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 2 | Kara Hanlon | 97 | Highland | 31.94 |
| 2 | 4 | Sarah Vasey | 96 | Derventio | 31.42 |
| 3 | 6 | Corrie Scott | 93 | Edinburgh Un | 30.64 |
| 4 | 8 | Ruta Meilutyte | 97 | Plymouth Lea | 29.92 |
| 5 | 7 | Sophie Taylor | 96 | London Aquat | 30.56 |
| 6 | 5 | Kathryn Johnstone | 90 | Edinburgh Un | 31.12 |
| 7 | 3 | Andrea Strachan | 92 | Edinburgh Un | 31.52 |
| 8 | 1 | Laura Kinley | 96 | Loughboro Un | 31.97 |