| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 0 | 1 | Sophie Smith | 94 | Loughboro Un | 27.54 |
| 1 | 3 | Charlotte Atkinson | 96 | Plymouth Lea | 27.29 |
| 2 | 6 | Emma Wilkins | 91 | Loughboro Un | 27.04 |
| 3 | 7 | Alys Thomas | 90 | Swansea Aq | 27.03 |
| 4 | 9 | Rachael Kelly | 94 | Loughboro Un | 26.57 |
| 5 | 8 | Rebecca Guy | 92 | Co Manch Aq | 26.99 |
| 6 | 5 | Raquel Matos | 96 | Edinburgh Un | 27.11 |
| 7 | 4 | Rachel Lefley | 90 | Co Manch Aq | 27.23 |
| 8 | 2 | Grace Vertigans | 96 | Loughboro Un | 27.54 |