| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 0 | 1 | James Wilby | 93 | Loughboro Un | 28.36 |
| 1 | 3 | Craig Benson | 94 | UniOfStirl | 28.32 |
| 2 | 5 | Euan Inglis | 93 | Edinburgh Un | 27.90 |
| 3 | 7 | Mark Tully | 92 | Edinburgh Un | 27.37 |
| 4 | 9 | Adam Peaty | 94 | Co Derby | 26.62 |
| 5 | 8 | Ross Murdoch | 94 | UniOfStirl | 27.28 |
| 6 | 6 | Andrew Weatheritt | 91 | Loughboro Un | 27.64 |
| 7 | 4 | Christopher Steeples | 92 | Co Manch Aq | 28.08 |
| 8 | 2 | Andrew Willis | 90 | Bracknell | 28.36 |