| Final | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 1 | Danielle Gilbert | 98 | Derwentside | 35.29 |
| 2 | 2 | Kate McQuaid | 98 | Hatfield | 35.06 |
| 3 | 3 | Annabel Guye-Johnson | 00 | RTW Monson | 34.63 |
| 4 | 4 | Sarah Tripp | 98 | Rushmoor Ryl | 34.17 |
| 5 | 5 | Gemma Kane | 98 | IRL | 34.17 |
| 6 | 6 | Holly Rowlinson | 01 | SwimTrafford | 34.93 |
| 7 | 7 | Madison Kemp | 98 | Poole | 35.23 |
| 8 | 8 | Jessica Shatford | 99 | Carnforth | 35.35 |