| Final | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 1 | Nicholas Grainger | 94 | Co Sheffield | 49.94 |
| 2 | 2 | Duncan Scott | 97 | UniOfStirl | 49.54 |
| 3 | 3 | Robert Renwick | 88 | UniOfStirl | 49.34 |
| 4 | 4 | Benjamin Proud | 94 | Plymouth Lea | 48.70 |
| 5 | 5 | Calum Jarvis | 92 | Bath Univ | 48.93 |
| 6 | 6 | Braxston Timm | 92 | Co Sheffield | 49.48 |
| 7 | 7 | James Guy | 95 | Millfield | 49.56 |
| 8 | 8 | James Disney-May | 92 | Millfield | 49.95 |