| Final | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 2 | Elise Bouwens | 91 | NED | 56.13 |
| 2 | 3 | Ruta Meilutyte | 97 | Plymouth Lea | 55.75 |
| 3 | 4 | Francesca Halsall | 90 | Loughboro Un | 55.51 |
| 4 | 5 | Femke Heemskerk | 87 | NED | 55.01 |
| 5 | 6 | Natalie Coughlin | 82 | Cal Aquatics | 55.31 |
| 6 | 7 | Ranomi Kromowidjojo | 90 | NED | 55.61 |
| 7 | 8 | Rebecca Turner | 92 | Co Sheffield | 56.11 |
| 8 | 9 | Emma Wilkins | 91 | Loughboro Un | 56.26 |