| Final | |||||
| Lane | Comp.No. | Name | YoB | Club/Country | Time |
| 1 | 2 | Amy Smith | 87 | Loughboro Un | 25.88 |
| 2 | 3 | Jeanette Ottesen Gray | 87 | DEN | 25.81 |
| 3 | 4 | Francesca Halsall | 90 | Loughboro Un | 25.51 |
| 4 | 5 | Ranomi Kromowidjojo | 90 | NED | 25.18 |
| 5 | 6 | Femke Heemskerk | 87 | NED | 25.43 |
| 6 | 7 | Natalie Coughlin | 82 | Cal Aquatics | 25.52 |
| 7 | 8 | Emma Wilkins | 91 | Loughboro Un | 25.88 |
| 8 | 9 | Elise Bouwens | 91 | NED | 26.07 |