| Place | Name | YoB | Club | Time | R.T. | FINA Pt | 50 | ||
| 1. | Jeanette Ottesen | (89) | Denmark | 54.14 | + 0.69 | 889 | 26.08 | ||
| 2. | Daniela Schreiber | (89) | Germany | 54.76 | + 0.82 | 859 | 26.29 | ||
| 3. | Hannah Wilson | (89) | Hong Kong | 54.89 | + 0.68 | 853 | 26.64 | ||
| 4. | Karin Prinsloo | (89) | South Africa | 55.06 | + 0.82 | 845 | 26.74 | ||
| 5. | Pernille Blume | (94) | Denmark | 55.28 | + 0.70 | 835 | 26.84 | ||
| 6. | Silke Lippok | (94) | Germany | 55.44 | + 0.70 | 828 | 26.89 | ||
| 7. | Lisa Vitting | (91) | Germany | 55.76 | + 0.72 | 814 | 26.81 | ||
| 8. | Ruta Meilutyte | (97) | Plymouth Lea | 56.26 | + 0.42 | 792 | 26.82 |